Living with Irresolute Cell Lines in an Automated World

An automated cell-culture platform becomes the nucleus of an organization performing cell-based research. However, every cell-based project placed on the system brings unique challenges. With each cell line comes millions of years of evolutionary encumbrance and a genetic inclination driving unique phenotypic peculiarities. In vivo, diverse eukaryotic cells rely on their “mammalian host” for survival. An automated system must perform in vitro, the myriad actions needed to sustain multiple cell lines as well, hence becoming an “automated host.” Cells invariably, will endeavor to do as they please. Molding these cells into the operational bounds of a man-made system requires insight into the relationship between cell and machine.

Citing our own experiences, we will describe herein the use of the SelecT automated cell-culture platform (The Automation Partnership, Hertfordshire, England) in our discovery and preclinical profiling programs at Novartis. Achieving the balance between cells and the automated environment, and accommodating variable cell dynamics are discussed.