A Microfluidic Cartridge System for Multiplexed Clinical Analysis

Cartridge-based microfluidics is a promising technology for clinical diagnostics. By miniaturizing the fluid-handling processes required for genomic and proteomic analyses, reagent and specimen volume is minimized along with the size of the system. We demonstrate an automated microfluidic system capable of performing six multiplexed genomic and proteomic analyses simultaneously, by means of an integrated electrochemical sensor and embedded controls.

Keywords: microfluidics, electrochemical sensor, multiplexed assay, quantitative, molecular analysis, point of care, clinical diagnostics

1 GeneFluidics, Inc., Monterey Park, CA

2 UCLA, Los Angeles, CA

3 University of California, Los Angeles, CA

4 University of Arizona, Tucson, AZ

5 Stanford University and Veterans Affairs Palo Alto Health Care System, Palo Alto, CA

6 David Geffen School of Medicine at UCLA, Los Angeles, CA

7 Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, CA

Correspondence: Vincent Gau, Ph.D., GeneFluidics, Inc., Molecular Analysis, 2540 Corporate Place, STE B101, Monterey Park, CA 91754; Phone: +1.323.269.0900; Fax: +1.323.269.0988

PII: S1535-5535(09)00102-6

doi:10.1016/j.jala.2009.05.002

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